Our sybr green qpcr protocol is a method designed to detect accurate quantification of gene expression and rt pcr reactions. The quantifast sybr green rt pcr kit delivers highly specific and sensitive results outperforming other real time rt pcr kits used in fast cycling mode see figure specific one step rt pcr rt pcr run times are reduced by up to 60 see figure significantly reduced pcr times allowing you to get results faster you can also greatly increase your sample throughput or efficiently share a. These master mixes are optimized for non roche qpcr systems for both sybr green reactions and probe based assays.
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