Kits include kapa sybr fast qpcr master mix 2x which contains kapa sybr fast dna polymerase reaction buffer dntps sybr green i dye and mgcl 2 at a final concentration of 2 5 mm where noted master mixes contain instrument specific reference dyes while the universal kit includes rox high and rox low both 50x separately. Fastreal time pcr results in as fast as 35 minutes sensit. Kapa sybr fast qpcr master mix 2x kits are a ready to use cocktail containing all components except primers and template for the amplification and detection of dna in qpcr.
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